IN-VIVO DOUBLED HAPLOID TECHNOLOGY IN MAIZE BREEDING Authors: Khakwani K , RAFIQUE M2, MALHI AR3, ALTAF M4 AND CENGIZ R5
ABSTRACT
This review of literature is aimed to summarize in-vivo maternal haploid inducer based doubled
haploid technology in maize breeding. The three crucial and important steps in this method
include the induction of haploids, their identification and subsequent duplication of these
haploids to produce doubled haploids lines. With the advent of haploid inducer lines, the
production and detection of haploids are possible in the dormant seed stage. The percentage of
spontaneous duplication in these haploid plants is very low and germplasm specific. Therefore,
the use of artificial duplicating agents is essential to increase the percentage of chromosomal
duplication. The cost of developing the DH line can be reduced with the detection of early
doubled haploids and the germplasm that supports spontaneous duplication. These developments
can replace the long laboratory procedures used for the development of the DH line.
Keywords: Doubled haploid, Zea mays, chromosomal duplication, inbred lines, maize breeding