IN-VIVO DOUBLED HAPLOID TECHNOLOGY IN MAIZE BREEDING
Authors: Khakwani K , RAFIQUE M2, MALHI AR3, ALTAF M4 AND CENGIZ R5

ABSTRACT
This review of literature is aimed to summarize in-vivo maternal haploid inducer based doubled haploid technology in maize breeding. The three crucial and important steps in this method include the induction of haploids, their identification and subsequent duplication of these haploids to produce doubled haploids lines. With the advent of haploid inducer lines, the production and detection of haploids are possible in the dormant seed stage. The percentage of spontaneous duplication in these haploid plants is very low and germplasm specific. Therefore, the use of artificial duplicating agents is essential to increase the percentage of chromosomal duplication. The cost of developing the DH line can be reduced with the detection of early doubled haploids and the germplasm that supports spontaneous duplication. These developments can replace the long laboratory procedures used for the development of the DH line. Keywords: Doubled haploid, Zea mays, chromosomal duplication, inbred lines, maize breeding

    https://ijbpas.com/pdf/2019/January/MS_IJBPAS_2018_4573.pdf
Download PDF
https://doi.org/10.31032/IJBPAS/2019/8.1.4573