ANTIMICROBIAL EFFICACY, PHYSICO- NUTRITIONAL PROFILE OF SESAMUM INDICUM L. SEED AND SEED OIL INDIGENOUS TO KPK, PAKISTAN
Authors: Ghaffar F . SHAH H AND KHAN I

ABSTRACT
The ever increasing dietary demands and the scarcity of edible oil sources have led to the need for the exploration of new sources in order to meet the local demands. Blessed with nature’s tremendous sources there is a dire need of exploring the non-traditional food and oil sources in Pakistan. The present study was undertaken to evaluate the physicochemical, nutritional, tocopherols’ composition and antimicrobial efficacy of the essential oils from two black Sesamum indicum seed locally grown in Khyber Pakhtunkhwa, Pakistan. About four fungal species (Phythium Ultimum, Paecilomyces Lilacinus ,Rhizopus stlonifer and Tricoderma Harzanium) and four bacterial strains (Xnythomonas campestris,Proteus Mirabilis, Escherichia Coli, Bacillus Cereus) were tested against different n-hexane and sesame oil fractions. Results showed good antimicrobial properties of the oils against all the pathogens. The proximate composition showed the seed cakes of both the varieties to be a good source of oil, protein, dietary fiber, ash. Both of the varieties showed to be good sources minerals. The intra variety difference in the nutrient composition was significant only in the percent oil, zinc, and copper. The physicochemical tests of the oils from both varieties revealed that refractive index, surface tension, density, specific gravity, saponification value, and iodine number fell well within the international standards for edible oils. The fatty acids composition showed the oils to be a rich sources of oleic acid, linolenic acid, and linoleic acid with an excellent proportion of unsaturated to saturated fatty acids. Intra variety differences were significant in oleic acid, elaidic acid, linoleic acid, and total unsaturated fatty acids. Tococpherol profile indicated the oils were rich sources of tocopherols though the variety S-17 appreciably greater proportion of ? ? and total tocopherols. These differences may be attributed to the genetic makeup or genotype response to the local climate. Both of the oils showed good DPPH free radical scavenging properties. The study conclude that indigenous variety of Sesamum Indicum possess excellent nutritional composition along antioxidant and antimicrobial potential making it ideal as food with utility in the development of variety of natural compounds. Keywords: Sesamum Indicum, Proximate and Elemental Composition, Tocopherol profile, physicochemical properties, Fatty acid Composition, DPPH free radical Scavenging, Antimicrobial activities.

    https://ijbpas.com/pdf/2018/April/MS_IJBPAS_2018_4396.pdf
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