ABSTRACT

Background Circulating miRNA-133a (miR133a) is a biomarker identified in various types of cancer. In this study, we evaluated the viability of miR-133a levels in oral sub-mucous fibrosis (OSMF) and oral squamous cell carcinoma (OSCC) in serum samples for screening in OSCC and OSMF patients. Methods The relative expression level of miR-133a was evaluated by quantitative real time RT-PCR (qRT-PCR) in the serum of 20 OSCC, 20 OSMF patients and 40 healthy subjects. Result The result of the study revealed the down-regulated expression of miR-133a in OSMF and OSCC patients as compared to healthy subjects. Conclusion miR-133a expression was lost in OSCC and OSMF tissues and hence it acts as a tumor suppressor marker in oral cancer along with higher stability, specificity, sensitivity and reliability. More studies are needed to validate it as an early diagnostic and prognostic biomarker in oral pre-cancer and cancer. Keywords: Micro RNA-133A, oral sub-mucous fibrosis (OSMF) and oral squamous cell carcinoma (OSCC)

https://ijbpas.com/pdf/2018/April/MS_IJBPAS_2018_4386.pdf

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