ABSTRACT

Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. Defects in insulin action and increased glucose levels in diabetes may lead to high lipoproteins in blood. Subsequent increase in lipids might lead to progression of diabetic complications.Persistent hyperglycemia leads to production of excess free radicals by non-enzymatic glycation of proteins, glucose oxidation and increased lipid peroxidation, resulting in impaired antioxidant defense and increased insulin resistance. Current antidiabetic therapy includes synthetic drugs that have undesirable side effects. Therefore, interest to find natural compound having anti-hyperglycemic, anti-lipase and antioxidant activities has increased which help in controlling diabetes and oxidative damages. Edible seeds can be a good option as they are rich in phytochemicals, having multiple health benefits including radical scavenging activities. In present study an effort was carried out to investigate in vitro antihyperglycemic, anti-lipase and antioxidant activities of methanolic extract of 2 edible seeds Salvia hispanica and Chenopodium quinoa. Preliminary phytochemical screening revealed the presence of alkaloid, flavonoid, phenol and saponins. S.hispanica exhibited highest phenolic (66.6±0.5 mg GAE/gm extract) and flavonoid (72.3± 0.8 mg QE/gm extract) contents. S. hispanica showed maximum DPPH and ABTS? scavenging activities (IC50=1.02 ?g/ml; 1.09 ?g/ml respectively). S.hispanica also exhibited maximum ?-amylase (IC50=1.13 mg/ml) inhibitory activity whereas C.quinoa showed maximum ?-glucosidase (IC50= 0.86 mg/ml) and moderate lipase (IC50=0.97 mg/ml) inhibitory activity compared to S.hispanica (IC50=1.37 mg/ml). Present results suggest that S.hispanica and C.quinoa seed extracts may have a great potential for further development as diabetes prevention agents or food supplements as a complementary pharmacological drug. Keywords: Salvia hispanica, Chenopodium quinoa, Antioxidant, Antihyperglycemic, Lipase inhibition

https://ijbpas.com/pdf/2018/March/1519918410MS IJBPAS 2018 4384.pdf

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