SCREENING OF ANTIOXIDANT ACTIVITY OF MARINE BACTERIA ISOLATED FROM MARINE SOIL OBTAINED FROM NORTH-WEST COASTAL REGION OF INDIA Authors: Harmalkar M , SHIVALE N, MARAR T, SAMANT M
ABSTRACT
The present study aims at enriching and isolating potential antioxidant producing
microorganisms from marine soil obtained from coastline of North-West coastal region of
India. A total of 156 bacterial isolates were obtained, which were screened for their potential
to produce extracellular antioxidants. Several non-pigmented and pigmented isolates obtained
which exhibited good antioxidant producing capabilities. Amongst the seven pigmented
organisms exhibiting superior antioxidant activity, two isolates viz. 6-3 and 8-1 demonstrated
a varied TLC banding pattern along with a significant DPPH scavenging activity. These
isolates were identified morphologically, biochemically and with 16S rRNA identification
studies as Janibacter melonis and Pseudomonas stutzeri respectively. The gene sequence of
both the organisms was submitted to NCBI and the strains were named Janibacter sp. TKB-1
and Pseudomonas sp. KKB-1 respectively.
Key words: Antioxidants, DPPH assay, TLC, Janibacter melonis, Pseudomonas stutzeri
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