ABSTRACT

Paracetamol is used as pain and fever treatment and listed as the most important medication needed in the basic health system. This study was established to separate paracetamol and some impurities from a mixture using high performance liquid chromatography (HPLC) via Ultra Violet (UV) detection. A gradient reverse-phase high performance liquid chromatography (RP-HPLC) with UV detector performed to detect paracetamol and impurities. The eluents consisted of water/methanol/ammonium acetate 95:5: 5m v/v/mM (eluent A) and Methanol/water/ ammonium acetate 95:5: 5 v/v/mM (eluent B) usinga gradient program at a flow rate of 300 ?L/min with 238 nm detection and an injection volume of 20 ?L. A fast and repeatable method for the separation by HPLC using reversephase (RP) was developed first. The impurities had been successfully separated. Keywords: Gradient; Impurities; Paracetamol; RP-HPLC

https://ijbpas.com/pdf/2018/March/1519918101MS IJBPAS 2018 4377.pdf

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