ABSTRACT

Gastro duodenal ulcer is a common disease caused by Helicobacter pylori. Amoxicillin and ranitidine are usually co prescribed drugs to treat such infections. An RP-HPLC method was developed and validated for the determination of amoxicillin and ranitidine in human plasma simultaneously. For this purpose, chromatographic system comprised of LC-10 AT VP pump and SPD-10 AVP UV/visible detector was used. The separation was best accomplished with mobile phase consisting of 0.02M phosphate buffer: acetonitrile (93:7 v/v, pH 3.0) from Hibar- Purospher star RP-18e (5 ?m, 250 x 4.6 mm) column at ambient temperature. The flow rate was 1.0 ml/minute and separation was carried out at 230 nm. The method was found linear over the concentration range of 0.085?g/mL to 20?g/mL for amoxicillin and 0.078?g/ml to 20?g/ml for ranitidine with correlation coefficient of 0.999. The retention times of amoxicillin and ranitidine were about 8 and 11 minutes respectively. The proposed method is accurate, precise, reproducible and economical. Keywords: Amoxicillin, Ranitidine, RP-HPLC, simultaneous, plasma

https://ijbpas.com/pdf/2018/January/1514894881MS IJBPAS 2017 4350.pdf

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